Good luck

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• Good luck!!!

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Hanoi University of ScienceHUS_ GIST Joint
training PhD program-----------------------

• Study Plan
• Student Phuc, hathi
• Back ground Biologist
• Center of Molecular Biology and Cell
  Technology

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• Subject of the research
• Applied and Environmental Biotechnology

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What are my motives ?

• Environmental Pollution is a big problem in the
  world
• In VietNam and many countries , it increases to
  an alarm level
• Using Biotechnologies in studying and remediation
  pollution is one of the most rapidly developing
  and eficiency fields in modern environmental
  technology
• I have an excellen background in Biotechnology
  with experience in Biochemistry, Microbiology and
  Molecularbiology researchs

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Titles of research plan

• A- Study the specific microrganisms,
  particularly bacteria, living in polluted
  environment at both physiology and genetic level
  and develop them for environmental remediation
  purposes
• B- Detection and monitoring of environmental
  pollutants as well as studying the effects of
  them on ecology by using Molecular Biotechnology

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• How to carry out ?

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A- Study the specific bacteria for
Bioremediation purposes

• The removal of pollutants from the environment
  via natural physico-chemical and biological
  processes (natural attenuation) is, in general, a
  slow and unpredictable way
• The most successful strategy to fight pollution
  is the use and manipulation of the detoxification
  abilities microorganisms, particularly bacteria
  ioremediation)
• The abundance of microorganisms, together with
  their great ability for horizontal gene transfer
  and their high growth rates, allows them to
  evolve quickly and to adapt to environmentally
  changing conditions, even to extreme environments
  that do not allow proliferation of other living
  organisms

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I Isolation and Selection

• Analyse the microbiological phases living in
  polluted invironment
• Isolate the specific bacteria capable of altering
  enviromentally toxic organic and inorganic
  compounds
• Select the best microorganisms giving effeciency
  Biodegradation of pollutant and toxic compounds
• Classification these microbes base on 16S r DNA
  or other biotechniques

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Colection
Isolation
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II- Analysis and Discovering the natural
phenomina of bacteria

• Research on Microbial physiology optimal growth
  condition ,enhancers, inhibitors, changes in
  growth process ...
• Mechanism of microbial degradation of pollutants
  and hazadous compounds,enzyme catalysis,
  enhancement, inhibition...
• Microbial genetics genetic characteristics
  genes are responsive to pollutants, genome
  maping...

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III- Genetic Engineering

• Detect and study structural genes and regulator
  genes envolve biodegradation process...
• Cloning and sequencing
• Decoding DNA or protein ,Recombination and
  influencing to regulation mechanisms in order to
  overcome the limitation of natural biodegradation
  process by microbes

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Genetic Engineering
Three Hardware Components
X
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Designing recombinant bacteria for bioremediation
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Applying for invironmental remediation purpose

• Develop of superbergs to degrade toxic materials
  and other pollutants in environment
• Applying accompany with correcting to reach
  hight efficiency in the reality
• Biotreatment must be combined wiht others
  techniques in environmental treatment process

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B-Detection and monitoring of environmental
pollutant using molecularbiotechnology

• Molecular techniques are powerful tools for
  monitoring environmental effects and
  characterizing microbial diversity
• Molecular tools such as rRNA probes, DNA
  extraction and analyses, i.e., denaturing
  gradient gel electrophoresis and microarrays, can
  effectively monitor changes in, and improve the
  understanding of microbial communities involved
  in vital ecosystem processes

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Molecular techniques

• Environmental biomornitoring for toxic chemical
  using biosesor and nanorbiosensor
• Using DNA chips to analyse environment pollution
• Identification of genes responsing in
  environmental stresses and toxic chemicals using
  DNA microarray
• Comparative analysis of responsive genes and
  their expression in environment with pollutant
  and toxic

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Goals of study and research

• Become highly competent scientist in
  Biotechnology Environment field
• Catching the fundimental knowledges and modern
  techniques in Environment remediation using
  Biotechniques
• Publications
• Apply and desseminate in VietNam, ensure a
  beauty country both for today and for the future

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• Thank you
• for your attention!