Chemical Digestion

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Chemical Digestion
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Digestion

• Definitions
• Enzyme
• Hydrolysis

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Indicates not a true enzyme
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Carbohydrate hydrolysis

• Starch amylose or amylopectin
• Hydrolysis
• In salivary gland salivary amylase
• In small intestine pancreatic amylase
• Digestion

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Testing for Carbohydrate Hydrolysis

• Lugols Iodine
• Interaction
• Controls
• Benedicts Test
• Benedicts tests for reducing sugars
• Reaction
• Green small amount of reducing sugars
• Yellow some reducing sugars
• Orange moderate reducing sugars
• Red large amount of reducing sugars

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Testing for Carbohydrate Hydrolysis
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Benedicts Test
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Protein Hydrolysis

• Proteins amino acids with peptide bonds
• Pepsin
• Function
• Small intestine
• Pancreas enzymes
• Trypsin and chymotrypsin
• Carboxypolypeptidase

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Testing for Protein Hydrolysis

• Biurets Test
• NaOH and CuSO4
• Reaction

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Lipid Hydrolysis

• Triglycerides
• Digestion
• Minute amounts by
• Lingual lipase
• Gastric lipase
• Small intestine.
• Fat
• BILE
• Emulsifier
• Reaction

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Testing for Lipid Hydrolysis

• Litmus
• Acid/base indicator
• Atmosphere
• Sudan IV
• Unsaturated fatty acids

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Testing for Lipid Hydrolysis