TGFb signaling

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TGF-b signaling
3-7-02

• Yin Sun
• Department of Biomedical Genetics
• Phone [phone]
• Room 9-3691

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TGF-b signaling
Mechanisms of signaling
Biology of TGF-b

• Identification of TGF-b
• Cloning of receptors
• Mechanism of receptor activation
• Smads
• Nuclear transcription control

• Growth inhibition/cell cycle control
• Promotion of tumor growth in vivo
• EMT
• Fibrosis

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Methods of exploration
genes
proteins
phenotypes
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Ann.Rev.Bio.Vol.67 p753
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Transforming Growth Factor b

• cooperate with transforming growth factor a to
  promote anchorage-independent growth of NRK
  cells.

De Larco Todaro PNAS Vol.84001
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TGF-b inhibits proliferation in vitro
Laiho etal. JBC Vol.2669108
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Binding of TGF-b in mutant mink lung epithelial
cells
Laiho etal. JBC Vol.26518518
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Complementation of ligand binding between mutants
Laiho etal. JBC Vol.2669108
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Cloning of receptors for TGF-b

• Biochemistry abundance
• Yeast two-hybrid disulfide bond, availability
• Expression cloning number of subunits
• Homology search Genbank lottery

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