KEMRI Polio Lab Report

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KEMRI Polio Lab Report
7th Inter-Country meeting of Directors of
poliovirus laboratories in the WHO-EMRO,The
Hashemite Republic Of Jordan
August 24-26, 2003
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Kenya Medical Research Institute
24 years of Excellence in Health Research
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KEMRI Polio LAB.

• Part of the Global Polio Lab. Network since 1995
• Designated National/Inter-Country Lab. Serving
  Kenya, southern Sudan, Somalia, Djibouti, Eritrea
  , the Seychelles and St. Hellena islands (THE
  HORN OF AFRICAN REGION).
• Support KEPI in Vaccine Potency Testing For
  (Measles, YF, Polio) and field surveillance
  
• Supported by KEMRI, WHO (HQs, AFRO EMRO), JICA
  and CDC

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EPI Lab. Management Structure
Lab. Director

Measles Lab. Manager
Polio Lab. Manager
Lab. Technical Supervisor
Measles Lab
Tissue Culture
Polio Lab.
Technologists , Engineer Technicians
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STAFF DISPOSITION

• CURRENT ACTIVE STAFF 100 IN POLIO LAB
• - One Research Officer ( Lab. Director)
  Dr. P. Borus
  
• One Research Scientist (Manager Safety Officer)
  New Staff
  
• Two Lab. Technologists
  Mr. P. Kingori
  
• 
  Mr.
  M.Nthiwa
  
• -One Senior Technician
  Mr Muchiri
  
• -Two Junior Technicians
  Ms J. Hassan and Ms E. Njeri
  
• An Institute Engineer
  Mr. Okondo
  
• Two Laboratory Assistants Mr.
  B. Mwangi and Ms.J. Lewett

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Internal Quality Control

• Cell Sensitivity testing
• Receive our cells (RD L20B) with low passages
  from NICD in South Africa Done Quarterly.
  
• Received NIBSC Polio Virus Reference Sabin
  strains.
  
• Proficiency Testing
• - WHO-AFRO 100 (July 2003)
• External Assessment by CDC WHO ( 2002)
• Regular Monitoring of Techniques
• Standard Operating Procedures (SOPs) 2003 and WHO
  Manual used
  

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Internal QC (3)

• Equipment
• Calibration of equipment done by Kenya Bureau of
  Standards
  
• Regular maintenance/servicing of equipment done
  by the Institute Engineer

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Networking

• Meetings with KEPI on a weekly basis to reconcile
  data and to discuss surveillance issues
  
• Relationship with WHO (WR, AFRO ,EMRO Hqs) good.
  WHO Provide us With the Lab.
  
• Supplies
• Assistance on data management
• Internet connection and air conditioner
• Useful exchange of ideas during AFRO meetings ,
  during accreditation or external assessment
  
• East Africa bloc Bulletins

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Important Operational Practices

• Monthly In-house meetings
• In-House computer Training in place.
• Weekly meetings with KEPI
• Recruited an Institute Engineer

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LABORATORY CONTAINMENT OF WILD POLIO VIRUS

• MANY TYPES OF CONTAINERS USED.
• WE HAVE REQUESTED FOR STANDARD STOOL CONTAINERS
  FROM GENEVA.
  
• STOOLS STORED IN ORIGINAL CONTAINERS.
• WATER PROOF TAPE STRAPPED AROUND THE CAP OF THE
  CONTAINER.
  
• ALL CONTAINERS PACKED IN ONE AUTOCLAVE BAG FOR
  EACH MONTH AND DISCARDED AFTER ONE YEAR.

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Results- 1999

• Country Sample Case P1 P2
  P3
  
• Somalia 82 41 4S 0
  2Wild
  
• Sudan 65 33 2S 0
  2Wild
  
• Djibouti 2 1 0
  0 0
  
• Total 149 75 6S 0
  4Wild

KENYA 490 245 14S 0
1S
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Surveillance Indicators -1999
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Results 2000

• Country sample Cases P1
  P2 P3
  
• Somalia 313 157 17S (73W) 26S
  15S (6W)
  
• Sudan 116 58 4S
  1S 0
  
• Djibouti 4 2 0
  0 0
  
• TOTAL 433 217 21S (73W) 27S,
  15S(6W)
  

KENYA 609 304 24S 10S
29S
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Surveillance Indicators 2000
KENYA 53.5 80.6 99.2
9.6
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Results 2001

• Country sample Cases P1 P2 P3
  NPENT
  
• Somalia 261 131 10 12
  20(13W)36 13.8
  
• Sudan 173 86 5(1W) 0 0
  24 13,9
  
• Djibouti 2 1 0 0
  0 0 0
  
• Eritrea 34 17 0 0
  0 5 7
  
• Total 470 235 15(1W) 13 20
  (13W) 65 11.7

KENYA 542 271 8 9 2
38 7.0
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SURVEILLANCE INDICATORS 2001
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Results 2002
Country sample Cases P1 P2 P3
NPENT Somalia 216 108 9
18 14(6W) 23 10.6
Sudan 175 88 0 0
0 29 16.5
Eritrea 72 36 0 0
0 8 11.0 Total 463
232 9 18 14(6W) 60
12.9
KENYA 506 256 2 4 3
32 6.3
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SURVEILLANCE INDICATORS 2002
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RESULTS (AUGUST)- 2003
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SURVEILLANCE INDICATORS -2003
100
0
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SAMPLES HANDLED BY KEMRI LAB 2000-2003
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PLAN OF ACTION- 2003

• Strengthen regular contacts between the Lab. With
  KEPI.
  
• To bring representatives from WHO-EMRO (Somalia ,
  Djibouti, Sudan) counterparts on board during our
  weekly meetings (Present once a Month).
  
• To identify contact persons in St. Hellena and
  Seychelles Islands.
  
• Training of 2 staff in NICD South Africa .

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Recommendations

• The number of countries supported and samples
  processed by the laboratory for EMRO has
  increased over the years- EMRO to consider more
  support to the lab in terms of
• Increasing the amount of money to USD 10,000
• ASSIST LAB. WITH TRANSPORT-VEHICLE.
• Training (Cell Culture /attachment of staff in
  their labs).
  
• Invitations to EMRO meetings encouraged.

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