Dirty Money

1
Dirty Money
Matt LarsonSimon Missy Boevers
Cory Haeffner Scott Suhr
INTRODUCTION The purpose of this experiment was
to observe the normal micro flora found on money
obtained from local establishments.

• MATERIALS AND METHODS
• Materials
• Money from five local banks and businesses
• Trypticase Soy Broth
• Hytachi 2406 Scanning Electron Microscope,
  dehydration solution, sputter coater
• Methods
• A subsample of bills were immersed in 25 ml of
  Tryp Soy Broth, and incubated at room temperature
  for four days.
• Samples were removed from broth and fumigated in
  37 formaldehyde fumes for 48 hours.
• Samples were dehydrated with a 5 to 100
  ethanol series finished with HMDS. Samples were
  exposed for five minutes solution except for 100
  and HMDS (ten minutes).
• Samples were air dried on microscope stubs and
  sputter-coated with gold.
• Images were taken using a Hitachi 2406 SEM in the
  SE mode.

Results
First National Bank, Waverly, 11k magnification
Hy-Vee, 1k magnification
Hy-Vee, 10k magnification
Pizza Ranch, Waverly, 10k magnification
First National Bank, Waverly, 1k magnification
Covenant Hospital, 10k magnification

• Conclusion
• Money does have a significant amount and
  diversity of micro flora