Title: Surface Enhanced Raman Scattering (SERS) Effect and Applications
1Mini Symposium on Surface Plasmons
Surface Enhanced Raman Scattering (SERS) Effect
and Applications
Cheng Guo 07/15/2008
21. Introduction to Surface enhanced Raman
scattering (SERS)
1920s discovery of Raman effect
1970s discovery of SERS effect of pyridine on
silver electrode surfaces
J. Am. Chem. Soc. 1977,99,5215
32. Theoretical explanations for SERS
- Electromagnetic field enhancement mechanism
- excitation of surface plasmon
- tends to form spacially localized hot areas
- the magnitude of enhancement 106- 107 times for
single colloidal silver, and 108 for the gap
between two coupled particles
- Chemical enhancement
- due to specific interactions, forming
charge-transfer complexes - the magnitude of chemical enhancement 10-100
times
4 3. SERS applications
trace analysis approaching single molecule
detection limit e.g. 100 pyridine molecules on Ag
electrode
classical electrochemical studies e.g. corrosion
processes, film growth, self-assembled monolayers
Efficient enhancing substrates Ag, Au and Cu,
rough surfaces or colloidal particles with the
size of tens of nanometers
surface enhanced anti-stokes Raman
scattering surface enhanced resonance Raman
scattering (SERRS)
biological samples e.g. DNA/protein detection
54. SERS related to our research
Example 1 C60 SERS signals
Vibrational spectroscopy, 2007, 43, 415
6(c)
(b)
(a)
How about use H2_at_C60 in this system?
7Example 2 multiplex SERS signals for DNA
detection
Science 2002, 297, 1536
8Multiplex SERS application four-color DNA
sequencing by synthesis
PNAS, 2008, 105, 9145
9Example 3 SERS single nanoparticle imaging
J.Phys.Chem. B, 2002, Vol. 106, 311
10 Detection of azido-dNTP for DNA sequencing
NNN asymmetric stretching at 2088 cm-1
Biospectroscopy 1998, 2, 233
11Summary
- Surface Enhanced Raman Scattering (SERS) has been
discovered for many decades and the surface
plasmon resonance theory helped explain the
mechanism - SERS was applied in many research fields. Current
study of SERS in C60, multiplex SERS for DNA
detection and AZT-dNTP SERS signals are useful to
our research