E. coli 3x6K Microarray Slide

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E. coli 3x6K Microarray Slide

• Produced by the Microarray and Proteomics
  Facility in the Department of Biological Sciences
  at the University of Alberta.

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Slide Basics

• Slide contains Operons Version 1 of 3 E. coli
  genomes
• Strains represented are K12, O157H7 (EDL933),
  and O157H7 (Sakai)
• 768 controls -12 positive and 12 negative each
  repeated 32 times
• Each spot in triplicate in non-adjacent arrays
• Printed on Corning Epoxide Slides for ease of use
  and low background signal
• Printed on GeneMachines OmniGrid 100 with
  Telechem SMP3 pins

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Pargon Dye Test
Paragon ss-DNA Binding Dye from Molecular Probes
shows presence of DNA on slide with much great
resolution and clarity. Actually confirms that
DNA is present on the slide. This is a standard
part of our QA/QC program.
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Paragon Dye cont-
Close up of Mid-group of spots shows excellent
detail on spot morphology and spot arrangement
after printing. Also shows spots that are buffer
controls as defined in the slide map.
Example of buffer control, there is not supposed
to be DNA here.
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Paragon Dye cont-
Close up of middle array from last slide shows
benefit of 200 um spot spacing. Spots are very
well defined with sizes ranging from 70 um to 110
um with excellent spacing between spots. Spots
are also very well formed and concentric as shown
in 3D image
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3x6K E.coli slide summary

• Genomes of 3 strain of E. coli represented in
  triplicate.
• Standard QA/QC test guarantee high quality slide
  from each print run.
• Slides stored/shipped in N2(g) environment in
  presence of desiccant and O2 scrubber to ensure
  high quality slides with long shelf life.
• QA/QC information, slide maps, slide images are
  available on-line from our website.
• www.biology.ualberta.ca/facilities/microarray