Introduction to the LSR II and FACSDiva 6.0 Software

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Introduction to the LSR II and FACSDiva 6.0
Software
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Four-laser, 10-color benchtop flow cytometer
left cover
right cover
side door
High Throughput Sampler
fluidics interconnects
control panel
SIP
power switch
Laser 488nm, 355nm, 405 nm, 633 nm
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Control Panel
Sample flow rates approximately 12, 35, and 60
µL/min ( LO, MED, and HI settings )
SAMPLE FINE ADJ
LO
MED
HI
RUN
STNDBY
PRIME
sample flow rate fluid control buttons
fluid control buttons
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Octagon Detector Arrays
longpass dichroic mirrors
bandpass filters
355 nm (UV) Indo-1, DAPI, Alexa Fluor
350 405 nm (Violet) CFP, Alexa Fluor 430,
Pacific Blue, Marina Blue, Alexa Fluor 405 488 nm
(Blue) FITC, PE, PerCP, Pe-Cy5 (aka
CyChrome), PI, PerCp-Cy5.5, PE-Cy7, GFP, PE-Texas
Red 633 nm (Red) APC, APC-Cy7, Alexa Fluor
647, Alexa 680
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488-nm blue laser
633-nm red laser
PerCP-Cy5.5
695/40BP 685LP
PerCP
PI
PE-Cy5
PE-Cy5
APC
670/14
660/20
FITC
635 LP
530/30
GFP
505 LP
488/10
SSC
735 LP
735 LP
575/26
780/60
780/60
PE
PE-Cy7
APC-Cy7
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355-nm UV laser
405-nm violet laser
Alexa405
DAPI
CascadeB
Pacific Blue
Alexa350
440/40
450/50
505 LP
505 LP
525/50
530/30
AmCyan
CascadeY
Indo-1 (Blue)
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To Login the computerLogin Name flow user
Password fccf
To start the software, double click the shortcut
icon on the desktop
shortcut icon
Login to FACSDiva Software
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FACSDiva workspace
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Preparing Sheath and Waste Containers
Fluidics Cleaning Each time you finish using the
cytometer, clean the sample injection tube and
the area between the injection tube and the outer
sleeve. This prevents the sample injection tube
from becoming clogged and removes dyes that can
remain in the tubing. CLEANING INSTRUCTIONS ARE
POSTED ON THE LSRII
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• LSRII Location
• BioSquare III, 670 Albany St. 5th Floor
• (Card access is required to enter the room)
• FCCF web address
• http//www.bu.edu/cores/flow-cytometry
• FCCF email
• flowcore_at_bu.edu
• Sorting facility phone number
• 617-414-5225