Selected and Differential Media

1
Selected and Differential Media
2
Define

• Selective Media
• Differential Media
• Enriched Media

3
Blood Agar Plate (BAP)

• Differential
• Enriched
• Use
• Growth
• Hemolysis
• Alpha
• Beta
• Gamma

4
C-CNA

• Selective
• Differential
• Results
• Gram ()
• Colistin
• Naldixic acid
• Blood
• Hemolysis

5
MSA

• Selective
• Differential
• Results
• Halotolerant (7.5 NaCl)
• CH2O Mannitol
• pH
• phenol red
• Fermentation
• Acid
• Yellow

6
MAC

• Selective
• Differential
• Results
• Gram (-)
• CV
• Bile Salts
• Lactose
• pH indicator
• Neutral red indicator
• Ferment pink/red
• No acid colorless

7
EMB

• Selective
• Differential
• Results
• Gram (-)
• Eosin dye
• MB dye
• Lactose
• Some acid pink
• Large acid green
• No acid colorless

8
Set Up

• One plate for each group
• Label quadrants as lab book indicates for each
  plate
• Cultures on cart
• Anaerobic Gas Pack
• BAP
• C-CNA
• All others aerobic
• 37C

9
BAP results

• Sheep blood enriched
• Growth most all microbes
• Fastidious
• Differential
• GP Hemolysins
• Hemolysis types
• Gamma
• Alpha
• Beta

10
Hemolysis
No hemolysis
11
C-CNA results

• Selective
• Growth GP
• Colistin
• Nalidixic acid
• Differential
• Hemolysis

12
MSA results
Selective 7.5 NaCl Staphylococcus
sp Differential Mannitol fermentation
phenol red ? yellow
13
MAC results

• Selective GN
• CV
• Bile Salts
• Differential
• Lactose fermentation
• NF colorless
• Coliforms
• Neutral red ? pink

14
EMB results

• Selective GN
• Dyes
• Eosin
• MB
• Differential
• Lactose fermentation
• NF colorless
• Acid production
• Small pink
• Large metallic green