Microbiology Unknown Lab BIO 187

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Microbiology Unknown LabBIO 187
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TSI agarTriple Sugar Iron Agar
0.1 dextrose 1.0 sucrose 1.0 lactose
A B C D E
(a) Red/red (no sugar fermentation) (b) Control
(c) Red/yellow (Glucose fermented but lactose
and sucrose not fermented) (d) Yellow/yellow
(Glucose fermented. Lactose and/or sucrose
fermented) (e) Red/yellow with H2S
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Penol Red broths
A B C D E
A (acid with gas)..B (acid)C (uninoculated
control)D (no recation) E (alkaline reaction)
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O-F tests
Control E. coli
Pseudomonas Alcaligenes
Fermenter Oxidizer
Nonsaccharolytic
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Motility Test
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Decarboxylation
uninoculated
Positive Negative
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Gelatin Hydrolysis
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Simmons Citrate
A B C
A PositiveEnterobacter B NegativeE. coli C
Control-uninoculated
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Phenylalanine Deaminase Test
neg pos
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Urea agar
pos uninoculated neg
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Urea Hydrolysis
Positive uninoculated
Negative
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Methyl Red Test
A B
A Positive (E. coli) B Negative (Enterobacter
aerogenes)
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Voges Proskauer Test
A B
A Negative (E. coli) B Positive (Enterobacter
aerogenes)
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Indole Test
Negative Positive
-
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Catalase Test
-
Positive Negative
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GRAM STAIN
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Urea Hydrolysis
Positive uninoculated
Negative
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• General Microbiology (BIOL 187) Laboratory Report
  (Unknown Identification)
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• Page 1 Cover page
• Include your name, the unknown number and you
  final Genus and species identification
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• Page 2 Test listing
• I want a () / (-) listing of each test in a
  vertical, easily scanned format
• Page 3 Discussion section
• Detail, in complete sentences, why your results
  justify your identification and rule out other
  possibilities.
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• Page 4 About My Microbe section
• Research your microbe and give me a several
  paragraphs about your microbe.
• (Don't copy word-for-word from your
  textbook---This will be frowned upon!)
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• Page 5 Dichotomous key
• Create a neat dichotomous key, which results in
  the correct separation of all 8 possible
  unknowns. This key should be legible and sized to
  occupy a complete sheet of paper (nothing
  microscopic is acceptable here)
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• Page 6Lab handout where you recorded your
  initial results