Third generation sequencing & Comparison of NGS Technology Platforms

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Third generation sequencing Comparison of NGS
Technology Platforms

• Sravanthi Joginipelli

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Available NGS technology platforms

• Three commercially available NGS platforms, i.e.
  Second generation sequencing technologies
• 1. Roche (454) FLX Genome Sequencer,
• 2. Illumina Genome Analyzer II
• 3. Applied Biosystems SOLiDTM (Sequencing by
  Oligo Ligation and Detection)
• Third generation sequencing platforms
• Helicos
• HeliScope
• Pacific Biosciences

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Third generation sequencing

• 3rd generation

• PacBio RS

• Single Molecule Sequencing Technology (tSMS)
• No amplification
• 10Gb os sequence data per 8 day run
• Single Molecule Real Time (SMRT) sequencing
  technology (PacBio RS)

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Roche illumina analyzers

• Genome Sequencer FLX

• Illumina Genome Analyzer

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Comparison Roche and Illumina
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Roche, Illumina and AB SoLiD
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Thank you
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References

• Dames S, Durtschi J, Geiersbach K, Stephens J,
  Voelkerding KV, Comparison of the Illumina Genome
  Analyzer and Roche 454 GS FLX for resequencing of
  hypertrophic cardiomyopathy-associated genes.
• DNA Microarray Inc, Genomic Services
  http//www.dnamicroarray.com/services_genomics_ngs
  .html
• Pacific Biosciences, http//www.pacificbioscience
  s.com/
• 454 lifesciences http//www.roche-applied-science
  .com/