Wet Mount PT: 2007A Specimen 1

1
Wet Mount PT 2007ASpecimen 1

• Evaluate micrographs 1-a, 1-b, 1-c and 1-d
  together as if different fields from the same
  patient
• Identify cellular images labeled as
• 1
• 2
• 3
• Enter results on result sheet

2
Micrograph 1-a Low Power
3
2
1
2
3
Micrograph 1b High Power
1
4
Micrograph 1c Low Power
3
1
5
Micrograph 1d High Power
2
3
2
3
6
Wet Mount PT 2007A Specimen 2

• Evaluate micrographs 2-a, 2-b, and 2-c together
  as if different fields from the same patient
• Identify cellular images labeled as
• 4
• 5
• 6
• Enter results on result sheet

7
Micrograph 2a Low Power
5
4
4
8
Micrograph 2b Low Power
4
5
5
4
9
Micrograph 2c Low Power
6
10
Wet Mount PT 2007A Specimen 3

• Evaluate micrographs 3-a, 3-b, and 3-c together
  as if different fields from the same patient
• Identify cellular images labeled as
• 7
• 8
• 9
• Enter results on result sheet

11
Micrograph 3a High Power
7
8
9
12
7
8
Micrograph 3b High Power
9
8
13
Micrograph 3c High Power
8
9
7
14
Wet Mount PT 2007A Educational Challenge

• Evaluate the following micrographs
• These will not be graded!

15
Gram Stain Squamous Epithelial Cell
16
Gram Stain Clue Cell
Nucleus
17
Gram Stain Agents of BV
Mobiluncus spp.
Gardnerella vaginalis
18
Gram StainSquamous Epithelial Clue Cell

• These images are not typical of what you see in a
  wet mount since you dont do gram stains (but
  allow you to visualize the differences between
  normal epithelial cells and clue cells)
• Squamous epi a few gram positive bacteria (blue
  cells, typical of lactobacilli) are seen on the
  surface of the cell
• Clue Cell The surface of the cell is almost
  totally covered with blue gram positive bacteria.
  The cell nucleus is seen as a small red dot.

19
Gram StainAgents of Bacterial Vaginosis

• Gardnerella vaginosis (small, gram negative red
  rounded rods - coccobacilli)
• Mobiluncus sp. (small, slender gram negative
  red curved rods).
• Both are normally found in the vagina (i.e.,
  normal flora)
• In bacterial vaginosis, these bacteria increase
  in number and degrade proteins in the vagina to
  form the amines that cause the characteristic
  unpleasant odor associated with BV.

20
Sign Date Result Sheet

• Testing Person
• Site Coordinator
• Send results to lab director within 10 days of
  electronic notification of PT challenge