Carbohydrate Identification Lab

1
Carbohydrate Identification Lab

• Results Conclusion

2
Results Which of the solutions reacted to
Benedicts Solution?

• Monosaccharide-Known
• Honey
• Apple Juice
• Milk (in some classes)

3
Results Which of the solutions reacted to
Iodine Solution?

• Polysaccharides
• Oat Water
• Rice Water
• Milk (a little bit)

4
Results Which of the solutions did not react at
all?

• Disaccharides
• Sugar Water

5
Conclusion (a) Mono, Di or Polysaccharides?

• Reaction with Benedicts ?Monosaccharides
  Present
• Benedicts only reacts with Monosaccharides
• Honey, Apple Juice and Milk contains
  monosaccharides
• You saw a orange-yellow-brown color change,
  resulting from Benedicts solution!

6
Conclusion(b) Structure of Molecules
Benedicts

• Reacted w/Benedicts ? Monosaccharide
• Monosaccharide 1 sugar
• Structure of Honey, Apple Juice Milk
• The carbohydrate was made of individual, single
  sugar units.
• Most likely glucose or fructose.

7
Conclusion(c) Mono, Di, or Polysaccharides?

• Reaction with Iodine ?Polysaccharides Present
• Iodine only reacts with Polysaccharides
• Oat Water, Rice Water Milk contains
  polysaccharides
• You saw a blue-black color change, resulting from
  Iodine reaction!

8
Conclusion(d) Structure of Molecules Iodine

• Reacted w/Iodine ? Polysaccharides
• Polysaccharide Many Sugars
• Structure of Oat Water, Rice Water Milk
• The carbohydrates were large molecules, made of
  repeating sugar units.
• Most likely starchy.

9
Conclusion(e) Mono, Di, Polysaccharides?

• No Reaction ? Disaccharide
• No Color Change Ever ? Disaccharide
• Sugar Water must contain disaccharides

10
Conclusion(f) Structure of Molecules Nada

• No Reaction ? Disaccharides
• Disaccharide 2 or Double Sugar
• Structure of Sugar Water
• Made of double sugar units
• Most likely sucrose or sucralose

11
Conclusion(g) Solutions Long Energy

• Oat Water Rice Water
• Largest Carbohydrates
• Most Bonds ? Most Energy over Long Period

12
Conclusion(h) Solutions Quick Energy

• Honey Apple Juice
• Small Carbohydrates
• Few Bonds ? Little Energy over Short Period