Tips to Perform Pipette Calibration

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Tips to Perform Pipette Calibration

• Pipettes are necessary equipment often used in
  chemical laboratories to measure and transfer
  specific amount of liquid.
• Pipettes are essentially narrow tube like
  equipment with a rubber bulb at the top.
• The tube is numbered from the top to the bottom
  mostly in increments of ten millimeters.
• Accuracy in pipette measurement is necessary as
  any discrepancy in reading can affect the result
  of chemical reaction.
• To ensure accuracy it is necessary to perform
  pipette calibration at regular intervals.
• Calibration of equipment is desirable even in the
  most advanced equipment as overtime several
  factors can affect the readings of measuring
  equipment.
• Calibration process helps to check whether or not
  the equipment is offering correct reading so that
  it can be rectified in time and efficiency of
  processes or laboratory test is maintained.

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Given below are instructions to help you perform
pipette calibration on your own.

• Things needed
• Beaker
• Erlenmeyer flask
• Pen and paper
• Pipette
• Pipette filler
• Thermometer
• Weight balance

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• 1.Clean the Pipette

• 2. Add Distilled Water

• Put some distilled water in an Erlenmeyer flask.
  Let it stand out on a bench for about 15 minutes.
  Now, measure the temperature of the water.

• Find out the mass of the beaker to the nearest
  tenth of a milligram, using a balance.

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• 3. Taking Help of Pipette Filler

1. Calculate and determine

• Now, using a pipette filler fill the pipette with
  the water from the flask. Move this water into
  the beaker. Weigh the beaker again. Note down the
  difference in weight of the beaker and calculate
  the mass of water discharged. Carry out this
  process three more times.

• Calculate and determine the mean of the four
  pipette measurements. 

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• 5. Adjusting the Buoyancy of Air

• 6. Calculating Density of Water

• Make an addition of 1.06 mg per gram to the mean
  mass to adjust for the buoyancy of air during the
  weighing. Do not follow this step in case you are
  using a digital scale.

• Calculate the density of water at the temperature
  you measured earlier in the process. Determine
  the mean volume of water discharged by the
  pipette using the formula Volume mass /
  density.

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• It is important to ensure your laboratory is
  clean and organized when carrying out
  calibration.  

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• You are not required to clean and dry the beaker
  following each of the four pipette measurements
  as doing this will only waste your time.

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• Take help of pipette calibration service provider

• Even after taking precautions and doing all the
  steps correctly, there are chances that your
  calibration process may not offer you precision
  in results. Besides, you may not like to use your
  valuable resources for this purpose. A great way
  to help you carry out pipette calibration work
  with accuracy is to take help of professional
  pipette calibration service providers. Certified
  agencies keep an array of sophisticated equipment
  and have a team of experience technicians to
  conduct calibration of equipment with precision
  and care, thereby helping you carry out
  laboratory tests with efficiency.

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For Further Info Visit www.RScalibration.com