RESULTS

1
Assessing the Sensitivity of Oligo Microarrays
When Studying Biodegradative Bacterial
Communities in the Environment.
Vincent J. Denef1,2, Bergholz P.1, Park J.1,
Hashsham S.A.1,3, Tsoi T.V.1, and Tiedje J.M 1
1 Center for Microbial Ecology and 3 Department
of Civil and Environmental Engineering, Michigan
State University, East Lansing 48824, MI, USA 2
Laboratory of Microbial Ecology and Technology,
Ghent University, Coupure Links 653, 9000 Gent,
Belgium
RESULTS
Comparison of substrates Results
Improving the method Overview
Comparison of substrates Results legend
Comparison of Substrates Summary Results
Comparison of substrates Hybridization _at_ 65 ºC
- 635 nm (LB400/RHA1 mixture)
Comparison of Labeling methods Methods
Cmt-Gaps
Superaldehyde
X1
X2
X4
X3
Legend Avg Mean of replicates Stdev
Standard Deviation of replicates Stdevn
Stdev / Avg n-7-4 amino-modified 70-mer _at_
40 micromolar spotting concentration
Comparison of Labeling methods Results
Sensitivity Analysis - Genome Dilution
Series Design
Legend Avg Mean of replicates Stdev
Standard Deviation of replicates Stdevn
Stdev / Avg Replicate 2 of ARES only 10
of data after filtering TSA signals gt
65000 (saturation) _at_ PMT 600
Sensitivity Analysis - Genome Dilution
Series Results
Sensitivity Analysis - Genome Dilution
Series Results
Notes 106 and 107 data from 2 replicate
experiments 5x106 data from 1 replicate
Each replicate provides 8 data points per
probe Bph genes bphA and bphC /
Plasmid genes fcbB and ohbB