Mouse Bone Nanoindentation Device

1
Mouse BoneNanoindentation Device

• Group Members Ryan Fischer
• Megan Toth
• Client Dr. Robert Blank, Ph.D., M.D.
• Advisor J.A. Gimm, Ph.D.

2
Outline

• Problem Statement
• Background Information
• Product Design Specification
• Design Alternatives
• Proposed Solution
• Future Work

3
Problem Statement

• The goal of this project is to adapt a MTS 858
  Bionix servohydraulic testing machine to indent
  mouse bone to determine the mechanical strength
  of bones.

4
Background Information

• Motivation
• Fragility Fractures
• Current Research
• Current Method
• Three-point bend test
• Nanoindentation
• Elastic Modulus

MTS Machine
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Nanoindentation
Indentation Schematic, 1
Load vs. Displacement, 1
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Product Design Specification

• Process to provide homogeneous surface for
  indentation.
• Stabilizer
• Attach to 5000 lb. Load cell with eight screws of
  3/8 fine thread, R2.375.
• Nanoindenter
• D0.01 mm
• Provide small indentation depth of 2-3 microns
  under load from 0-1 N.
• MTS Attachment
• Attachment for Nanoindenter to Load Cell

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Design Alternatives

• Process for Smoothing
• Stabilizer
• Piece Model
• Nanoindentor tip
• Square Pyramid
• Nanoindenter Attachment
• Double Jointed

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Smoothing Process

• Fix bone in Petri dish with epoxy.
• Stabilize Petri dish in epoxy.
• Saw bone with microscopy saw.
• Polish bone with serial diamond cloths of
  increasing fineness placed over the saw blade.

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Piece Model
5,000 Lb. Load Cell
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Square Pyramid
Bottom View
Side View
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Double-Jointed
50 Lb. Load Cell
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Proposed Solution

• Smoothing Procedure
• Screw Model for Stabilizer
• Trigonal Pyramidal Indentor Tip
• Straight Indenter Attachment

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Screw Model
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Trigonal Pyramidal Indenter
Bottom View
Side View
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Straight Indenter Attachment
50 Lb. Load Cell
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Future Work

• Research on
• Indenter Tip Materials
• Load Cells
• Stabilizer Materials
• Develop Final Design
• Build Prototype
• Testing

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Acknowledgements

• Dr. Robert Blank
• Ron McCabe
• Tony Escarcega
• 1 http//www.inano.dk/sw262.asp