Molecular diagnostics

1
Molecular diagnostics
2
Immunological detection system(Sensitive,
specific and simple)

• 1
• 2
• 3
• 4
• 5

3
1
Enzyme-Linked Immunosorbent Assay
2
Polyclonal Ab
3
Polyclonal Ab drawbacks 1.Definition 2.The
amount of different Ab in a polyclonal Ab vary
from betch to batch 3.can not distinguish between
two similar targets (both generate signals)
4
monoclonal
polyclonal
Ag
(cross-reacted)
??B???????? Less Ag specific Specific for
poly-epitopes of an Ag Band pattern
cross-reacted More sensitive Ab-Ag affinity
avidity ??? ? ???
??B????????? More Ag specific Specific for
mono-epitope of an Ag Band pattern clear Less
sensitive Ab-Ag affinity avidity ???