Taking hops beyond the brewing industry

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Taking hops beyond the brewing industry

• William Popa
• Beta Tec hop products

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CO2 Extract is separated into the following
fractions at the ISO- Plant
Essential Oils Flavor Foam Color
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Where are Hop Acids being used?
Hops
2o
1o
Hop Extract
1o
2o

Alpha Acids
Beta Acids
Brewing Products
Ethanol Spirits
Sugar
Animal Feed
Nutraceutical
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Effects of Hops on the Bacterial cell wall
Hop acids lower the cell ph and stop the glucose
uptake
Normal Bacterial cell wall
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Results IsoStab StudyPart 1

• Prepared by Biotechnology Resource Center
  University of Minnesota
• for
• BetaTec, Inc.
• May 5, 2005

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Minimal Inhibitory Concentrations of IsoStab
against Lactobacillus species and
Ethanol-Production strains of Saccharomyces
cerevisiae

• It is clear that the growth of all three strains
  of Lactobacillus are inhibited by 6ppm of
  IsoStab. At the recommended concentration of
  hop acids to be used during ethanol production
  from corn mash (30ppm) the Lactobacillus species
  chosen are inhibited from 75-91.

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The Effects of High Concentrations of IsoStab and
Yeast

• The data presented shows that up to 200 ppm of
  IsoStab has virtually no effect on the growth of
  the six yeast strains. At 300ppm to 400 ppm, a
  slight reduction in growth can be observed and at
  500ppm, at least 2 strains show a significant
  reduction in growth.

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Data Page from In-Plant Testing on Corn
MashFebruary 2005
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Summary of IsoStab Batch Plant Test Data March
2005

The corn mash had a pH of 5.8. Some yeasts will
increase Lactic acid and glycerol levels when
they are in a healthy state.
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Citric Byproduct, Molasses and Sugar Waste
Process
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Summary of IsoStab Batch Plant Test Data February
2006
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Summary of IsoStab Batch Plant Test Data February
2006
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Conclusion The results from this trial are very
positive and indicate that IsoStab is an
effective product to inhibit lactic acid and
acetic acid formation without affecting yeast.
In-fact, yeast during the treatment was healthier
as indicated by higher viability numbers through
out the fermentation train.
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Linda Benjamin, PhDFDA/Center for Veterinary
Medicine
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IsoStab The natural antibacterial control
agent

• IsoStab is a standardized 30 aqueous alkaline
  solution of
• isoalpha acids. Patent pending for IsoStab.
• IsoStab can effectively control the growth and/or
  kill gram-
• positive bacteria commonly found in fermentable
  solutions
• such as Lactobacillus brevis and Lactobacillus
  fermentum.
• IsoStab is food grade GRAS FDA and is Kosher
  certified
• can be used to make spirits, ethanol and food
  grade yeast.
• IsoStab is very soluble and is ideal for dry-mill
  fermentations
• containing high solids.

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Normal Treatment Range for IsoStab in Corn Mash
at a Batch Plant is 30 - 60 ppm

• Lactic Acid Concentration
• 0.01 To 0.15
• 0.15 To 0.25
• 0.20 To 0.35
• 0.35 To 0.45
• 0.45 To 0.70

• IsoStab Treatment Levels
• 10 to 35 ppm
• 30 to 45 ppm
• 35 to 45 ppm
• 45 to 55 ppm
• 60 to 90 ppm

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What is possible when you use IsoStab ?

• Your plant efficiency will increase
• Your Acetic and Lactic acid level will be
  controlled
• IsoStab is Kosher Certified Gras / FDA
• Hops are listed in the AAFCO as a feed
  ingredient
• Your DDG will be antibiotic free
• You can sell your DDG to Europe, McDonalds,
  Burger King and to the Chicken Industry in the
  United States.
• IsoStab is a Yeast enhancer
• Faster fermentations

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What would be the impact on your DDG if you used
IsoStab?

• John I Haas, Inc. has be granted the patent for
  Hop Acids to be used in cattle feed
• A method of using hop acids for increasing
  food and energy uptake from feed by livestock
  Hops increase the production of propionate and
  lactate and decreasing the production of methane

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Today in the United States Ethanol Industry

• Beta Tec has over 45 customers using IsoStab
  daily
• Both continuous and batch plants using IsoStab
• Beta Tec is currently adding new technical sales
  positions
• For more information www.bthp.info
• Contact William Popa (Bill) at 816-509-0200

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Plant Tours
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