ChemBiol 473

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Chem/Biol 473 S. Schultze and G. Prody
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Figure 5-21 The central dogma of molecular
biology.
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How do RNA and DNA differ?
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Figure 5-6 Bacteriophages attached to the surface
of a bacterium.
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Figure 5-7 Diagram of T2 bacteriophage injecting
its DNA into an E. coli cell.
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Figure 5-8 The Hershey-Chase experiment.
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• You need to be able to draw and name
• their respective nucleosides and
• the nitrogen containing bases (what are they)
• their respective nucleotide mono-, di- and
  triphosphates

You also need to remember amino acid structures,
HH, MM kinetics, thermodynamics, etc. from 471,2.
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Figure 5-1 Chemical structures of (a)
ribonucleotides and (b) deoxyribonucleotides.
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Table 5-1 Names and Abbreviations of Nucleic Acid
Bases, Nucleosides, and Nucleotides.
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Figure 5-2 Chemical structure of a nucleic acid.
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(No Transcript)
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Figure 5-19 Electron micrograph of a T2
bacteriophage and its DNA.
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Figure 29-17 Electron micrographs of circular
duplex DNAs. Their conformations vary from no
supercoiling (left) to tightly supercoiled
(right).
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Figure 29-24 Agarose gel electrophoresis pattern
of SV40 DNA.
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Table 5-2 Sizes of Some DNA Molecules.
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Figure 5-14 Schematic representation of the
strand separation in duplex DNA resulting from
its heat denaturation.
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Figure 5-15 UV absorbance spectra of native and
heat-denatured E. coli DNA.
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Figure 5-16 Example of a DNA melting curve.
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Figure 5-17 Variation of the melting
temperatures, Tm, of various DNAs with their G
C content.
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Figure 5-10 X-ray diffraction photograph of a
vertically oriented Na DNA fiber in the B
conformation taken by Rosalind Franklin.
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Figure 5-11 Three-dimensional structure of B-DNA.
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Figure 5-12 Watson-Crick base pairs.
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Figure 29-1a Structure of B-DNA. (a) Ball and
stick drawing and corresponding space-filling
model viewed perpendicular to the helix axis.
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Figure 29-1b Structure of B-DNA. (b) Ball and
stick drawing and corresponding space-filling
model viewed down the helix axis.
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Figure 29-2a Structure of A-DNA. (a) Ball and
stick drawing and corresponding space-filling
model viewed perpendicular to the helix axis.
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Figure 29-2b Structure of A-DNA. (b) Ball and
stick drawing and corresponding space-filling
model viewed down the helix axis.
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Figure 29-3a Structure of Z-DNA. (a) Ball and
stick drawing and corresponding space-filling
model viewed perpendicular to the helix axis.
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Figure 29-3b Structure of Z-DNA. (b) Ball and
stick drawing and corresponding space-filling
model viewed down the helix axis.
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DNA tutorial link http//molvis.sdsc.edu/dna/inde
x.htm
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Figure 29-7 The conformation of a nucleotide unit
is determined by the seven indicated torsion
angles.
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Figure 29-8 The sterically allowed orientations
of purine and pyrimidine bases with respect to
their attached ribose units.
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Figure 29-10b Nucleotide sugar conformations.
(b) The C2?-endo conformation, which occurs in
B-DNA.
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Table 29-1 Structural Features of Ideal A-, B-,
and Z-DNA.
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Figure 5-13 Demonstration of the
semiconservative nature of DNA replication in
E. coli by density gradient ultracentrifugation.
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Figure 5-31 Action of DNA polymerases.
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Figure 5-32a Replication of duplex DNA in E. coli.
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Figure 5-32b Replication of duplex DNA in E. coli.
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