Experiment 4 Gene Expression Study in Arabidopsis Thaliana

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Experiment 4- Gene Expression Study in
Arabidopsis Thaliana
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Tools For Studying Differential Gene Activity
Microarrays
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What Are The Major Differences Between DNA and
RNA?
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RNA Has a Uracil Instead of Thymine
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RNA Has Ribose Sugar in Nucleotide
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Eukaryotic RNAs Are Single-Stranded
Polynucleotides
Nucleotides Joined By Phosphodiester Bonds Like
All Nucleic Acids
Order 5 ? 3 Leads to FunctionCo-Linear With
Gene Sequence
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RNA Has Intra-Strand Double Helices or Secondary
Structure
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Total RNA Isolation Workflow
1. RNA Isolation
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Determining Quality of Isolated Total RNA Using A
Capillary Gel Electrophoresis System
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Determining Quality of Isolated Total RNA Using A
Capillary Gel Electrophoresis System
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Determining Quality of Isolated Total RNA Using A
Capillary Gel Electrophoresis System- Part II
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