Phylogeography of the streamline chub Erimystax dissimilis

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Phylogeography of the streamline chub Erimystax
dissimilis

• (Actinopterygii Cyprinidae)

Rene Wolfe, Nick Gidmark, Ryan Peterson
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Phylogeography

• Field of study that looks at the geographic
  distributions of genotypes and attempts to
  account for the spatial patterns of disjunct taxa

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Distribution of E. dissimilis
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Sampling sites
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Collecting E. dissimilis
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Experimental questions

• What are the relationships of E. dissimilis
  populations?
• What are the patterns of haplotype diversity?

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METHODS

• Sequenced cytochrome b
• Parsimony analysis
• Bayesian analysis

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Map Cytochrome b
L14724
H15915
Amplified using HA, HE, LC, LA, H15915, L14724
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ANALYSIS

• Sequenced 51 individuals and added them with the
  data set of 85 others for a total 136
• Analyzed a 780 bp sequence of the 1140 bp
  cytochrome b gene in PAUP and Mr.Bayes

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Majority Rule
CLADE A
CLADE B
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RESULTS

• All systems were once interconnected and had gene
  flow
• Populations of Erimystax dissimilis are most
  likely segregated due to destroyed habitat by
  dams, impoundments, etc.
• It is hypothesized that a Green River haplotype
  dispersed out before river system changed
• The Green River haplotype occurs in low frequency
  outside of the Green River, only one individual
  has been collected

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To come..

• Sequence all individuals for nuclear S7 to
  further explore E. dissimilis haplotypes patterns
  
• Reamplify and sequence the Green River individual
  nested in clade A, and also reamplify and
  sequence the beginning and end of cytochrome b to
  recover trimmed bases

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Acknowledgements
Andrew Simons Brett Nagel NSF U of M Ichthyology
Lab