Slide sem ttulo

1
Bioinformatics for Genomics
8 seqs/day
96 seqs/2 hrs
2
A
G
C
T
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A
TAGAGCATCGATCGATGCTGCAGATGATGCTAGCATCGGCTAGGCGACG
3
Base calling trimming
chromatogram
acgatctcgctagctgctactgtagccgcgattattcgcgatctacgtat
atcgcgatcgatc

• Phred does the base calling

• Each base has assigned a chance of failure

• 1 0,01 10-2 Phred 20

• Random base calling at the beggining or the end
  of read (Phred lt 10)
• Trimming (trim or trim_alt algorithms)

4
Start
End
5
Partial sequencing of transcripts in EST genome
projects

• Goal
• To document the presence of transcripts in a
  transcriptome otorrin... in portuguese
• EST Expressed Sequence Tag

actgatcatctcgctgatgcgatc
work