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Detection%20of%20QTL%20in%20beef%20cattle

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Detection of QTL in beef cattle. Eduardo Casas. U.S. Meat Animal Research Center, ... Quantitative trait loci (QTL) on bovine chromosome 5. Identification of ... – PowerPoint PPT presentation

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Title: Detection%20of%20QTL%20in%20beef%20cattle


1
Detection of QTL in beef cattle Eduardo
Casas U.S. Meat Animal Research Center, Clay
Center, Nebraska
2
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3
Microsatellites and Single Nucleotide
Polymorphisms
Microsatellites
SNP
Alelle A
CCGTATCACACAGTCAAC
GAACTAGCTTGAC
Alelle B
CCGTATCACACACACAGTCAAC
GAACTGGCTTGAC
4
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5
Bovine Chromosome 2 (BTA2).
(Kappes et al., 1997)
6
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7
(No Transcript)
8
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9
Identification of QTL in cattle What have we
used to identify QTL? What have we
found? Where are we validating them?
10
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11
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12
Family from 906802 (Bonzo)
X
X
n 500
13
Family from 906801 (Blackie)
X
X
n 500
14
¼ Hereford
¼ Angus
¼ Pinzgauer
¼ Red Poll
MARC III
http//www.ansi.okstate.edu/breeds/index.htm
15
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16
Identification of QTL in cattle What have we
used to identify QTL? What have we
found? Where are we validating them?
17
GENOME SCAN RESULTS IN ALL RESOURCE FAMILIES
Blackie
BM
Bonzo
PA
BTA2
BTA1
BTA3
BTA4
BTA5
MSTN
RPYD, FATYD
WBS14
FAT, YG, RPYD, WBS14 RIBBONE, DP, RIBFAT, BWT
RPYD FATYD
MARB, RPYD MARB
MARB, RPYD MARB, RPYD Fat yield
HCW, WBS3
BTA8
BTA15
BTA27
BTA29
BTA6
FAT, MARB FAT
BWT, W365, HCW, LMA
WBS14
MARB Dairy Form
WBS3, WBS14, Calpain
18
Identification of QTL in cattle What have we
used to identify QTL? What have we
found? Where are we validating them?
19
Germplasm Evaluation, Cycle IV
Dam line
(n 71)
(n 109)
Sire line
(n 6) 52
-------
(n 7) -------
96
(n 35) 35
41
PH
(n 5) 52
-------
PA
(n 5) -------
47
Total
(n 6) 117
184
20
Germplasm Evaluation, Cycle V
Dam Hereford MARC III Angus
F1
BB(F1)
Ch(F1)
n 1551 (from 1998 to 1999)
(Cundiff et al., 1998, 1999, 2000)
21
Germplasm Evaluation, Cycle V
F2 subpopulation
n 154 (from 1998 to 1999)
(Cundiff et al., 1998, 1999, 2000)
22
Germplasm Evaluation, Cycle VI
Dam Hereford MARC III Angus
Sire Hereford Angus Wagyu Norwegian Red Swedish
Red White Friesian
F1
(N 415)
Ch(F1)
n 820 (from 2001 to 2002)
(Cundiff et al., 1998, 1999, 2000)
23
Germplasm Evaluation, Cycle VII
Sire Hereford, Angus, Red Angus, Limousin, Ch
arolais, Simmental, Gelbvieh
Dam Hereford MARC III Angus
F1
n 1400 (from 1999 to 2001)
Approximately 600/year (from 2003 and on)
F2
(Cundiff et al., 1998, 1999, 2000)
24
Germplasm Evaluation, Cycle VIII
Sire Hereford Angus Beefmaster Brangus Bonsmara
Romosinuano
Dam Hereford MARC III Angus
F1
n 1200 (from 2001 to 2002)
Terminal cross
(Approximately 600/year)
(Cundiff et al., 1998, 1999, 2000)
25
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26
Calving Ease Selection (CE)
Heifer Calving Difficulty
Birth Weight EBV, lb
27
Characterization of the variation of Myostatin
(BTA2) in resource populations.
28
Characterization of variation for QTL on BTA5 for
carcass traits in resource populations.
29
Characterization of variation for QTL on BTA6 for
growth and carcass traits in resource
populations.
30
Characterization of variation for QTL on BTA27
for marbling in resource populations.
31
Characterization of variation for QTL on BTA29
for meat tenderness in resource populations.
32
Characterization of QTL variation in resource
populations.
PA
BM
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