Microscope

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Introduction to the Microscope

• What is it?
• Care
• Parts
• Focusing

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The Light Microscope

• Compound microscope uses compound lenses and
  light to magnify objects
• The lenses bend or refract the light, which makes
  the object beneath them appear closer
• Hans and Zacharias Janssen are credited with
  creating the first microscopes
• Robert Hooke and Anton van Leeuwenhoek were
  pioneers in early microscope development

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Microscope Care

• Always carry with 2 hands (one hand on the arm,
  one on the base)
• Only use lens paper for cleaning
• Do not force knobs
• Keep cords away from sinks due to risk of
  electrical shock

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Microscope Parts
Eyepiece
Body Tube
Revolving Nosepiece
Arm
Objective Lens
Stage
Stage Clips
Coarse Focus
Diaphragm
Fine Focus
Light
Base
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Using the Microscope

• Place the Slide on the Microscope
• Use Stage Clips
• Click Nosepiece to the lowest (scanning)
  objective
• Look into the Eyepiece and adjust the slide until
  object is centered
• Use the Coarse Adjustment Knob to bring into
  focus
• When observations are completed (at this
  magnification,) proceed to adjust to Low Power.

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Using High Power

• When High Power is needed, make sure the object
  is centered and focused at scanning and low power
• Click the nosepiece to the high power objective
  (largest) lens
• Do NOT use the Coarse Focusing Knob
• Use the Fine Focus Knob to bring the slide into
  focus
• Never remove a slide while under high power

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Magnification

• Your microscope has 3 magnifications Scanning,
  Low and High objective lens
• Each objective lens will have written the
  magnification. In addition to this, the ocular
  lens (eyepiece) has a magnification
• The total magnification is the eyepiece times the
  objective lens

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Troubleshooting

• Q Image is too dark!
• A Adjust the diaphragm, make sure your light is
  on
• Q There's a spot in my viewing field, even when
  I move the slide the spot stays in the same
  place!
• A Your lens is dirty. Use lens paper, and only
  lens paper to carefully clean the objective and
  ocular lens
• Q I can't see anything under high power!
• A Remember the steps, if you can't focus under
  scanning and then low power, you won't be able to
  focus anything under high power
• Q Only half of my viewing field is lit, it
  looks like there's a half-moon in there!
• A You probably don't have your objective fully
  clicked into place

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