Dr' J'K' Pal

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A MOLECULAR DIAGNOSTIC FOR ANAEMIA, HEAVY METAL
TOXICITY HEMATOLOGICAL DISORDERS

Dr. J.K. Pal Department of Biotechnology,
University of Pune email jkpal_at_unipune.ernet.in
Phone [phone]
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ANAEMIA
DEFINITION Reduction in the functional mass of
circulating red blood cells is termed anaemia and
it results in the reduction of blood haemoglobin
level
CAUSE The 3 most common causes are, a) blood
loss, b) increased rate of destruction of
RBC, and c) impaired production of RBC
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Currently, diagnosis of progressive ANAEMIA is
made by observing increasing numbers of
RETICULOCYTES in the blood
Control
Peripheral blood smear showing increase in
reticulocyte during drug- induced anaemia in
rabbit
During progression of anaemia
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HRI, A MOLECULAR MARKER FOR DRUG-INDUCED ANAEMIA
(Western blot)
acute anaemia during recovery
during recovery
1 control 2-4 anaemic
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RETICULOCYTE COUNT VS HRI WESTERN BLOT
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2
3
4
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HRI AS A MARKER FOR LEAD-TOXICITY (PCR DETECTION
OF HRI EXPRESSION IN HUMAN K562 ERYTHROID CELLS)
B - (1, marker DNA 2, control 3-5, exposed to
100 ug/ml lead for 2,8 and 16h, respectively)
A - (1, marker DNA 2, control 3-5, exposed to
lead acetate conc. of 10,100 and 500 ug/ml,
respectively)
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DIP-STICK METHOD FOR PROGNOSTIC DETECTION OF
ANAEMIA
CURRENT WORK IN PROGRESS 1. PURIFICATION OF
HUMAN HRI 2. PREPARATION OF ANTI-HUMAN
HRI MONOCLONAL ANTIBODIES 3. SCREENING OF BLOOD
SAMPLES BY WESTERN BLOT AND RT-PCR 4.
DESIGNING OF DIPSTICK KIT
NEGATIVE CONTROL
HRI VE TEST SAMPLE
HRIVE STANDARD
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POTENTIAL APPLICATIONS OF THE HRI DIPSTICK
USER FRIENDLY
RAPID (1.5H) EASY ALTER-NATIVE TO EXISTING TESTS
ABILITY TO MONITOR SUCCESS OF DRUG THERAPY
HIGHLY SENSITIVE
MULTIPLE APPLICATIONS (DRUG- AND HEAVY METAL-
TOXICITY, LEUKEMIAS, MALARIA, LEISHMANIASIS)
MINIMUM LAB INFRASTRUCTURE OR EQUIPMENT NEEDED
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OTHER RESEARCHERS IN THE TEAM 1. Dr. Sanjay
Anand 3. Mr. Angshuman Sarkar 3. Ms. Sunita
Shankar Financial support CSIR UGC
DBT Publication Recognition 1. J. Biosci.
22, 287-298 (1997) Received Best paper of 1997-
A. Krishnamurthy Award from The Society of
Biological Chemists, India. ((