Lyme Disease

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Lyme Disease Vibramycin

• Presented by Emily McVey

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Lyme Disease

• First discovered in Lyme, Connecticut in 1975
• Diagnosed as juvenile Rheumatoid Arthritis in
  children
• Incubation period takes 1-2 weeks
• Spread by the deer tick (Ixodes scapularis)
• Borrelia Borgdorferi

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Borrelia Borgdorferi

• Bacterial spirochete
• Reservoirs- rodents, deer, and ticks
• Vector borne- spread through the bite of ticks

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Acute Symptoms

• Bulls-eye rash (which appears at the bite site)
• Acute Symptoms
• Backache
• Fatigue
• Chills
• Headache

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Chronic Symptoms

• Arthritis
• Neurological impairment (weakness of limbs and
  facial ticks)
• Cardiac damage
• Visual disturbances
• Facial paralysis
• Seizures
• Stiff neck and severe headache

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Misdiagnosis

• Acute
• Rheumatoid Arthritis
• Flu
• Sinusitis
• Fibromyalgia

• Chronic
• Bells Palsy
• Meningitis
• Chronic Fatigue Syndrome
• Multiple Sclerosis

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Diagnosis

• Antibody testing
• ELISA (enzyme-linked immunosorbent assay)
• Western Blot
• Outer surface protein (OSP) C

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Treatment

• Vibramycin (Doxycycline)

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Doxycycline

• Doxycycline is in the Tetracycline family
• Inhibits protein synthesis? translation
• Selectivity

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Pharmacokinetics

• Passes through outer membrane through porins
• The passage through the inner membrane depends on
  a pH gradient
• Inhibits protein synthesis by binding to the 30S
  subunit of ribosomes and preventing aminoacyl
  tRNA from binding (stops further addition of
  amino acid to growing proteins)

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Pharmacokinetics

• Doxycycline mimics the aminoacyl group, which
  prevents the binding of the aminoacyl and the
  tRNA group

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Elimination

• Once Doxycycline is metabolized by the liver, it
  is excreted through the urine

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Still looking in to

• How (specifically) the medicine gets into the
  bacterial cell (the inner membrane)