Biacore 2000 Maintenance protocols

1
Biacore 2000 Maintenance protocols
.Desorb
Who? All users are responsible for running
desorb during their booking period. When? Desorb
should be run at least once every week. For
certain samples it might be necessary to run
desorb more frequently, i.e. after the
experiments are finished. You should try to
avoid running desorb in the middle of your
experimental session as results before and after
a desorb might be inconsistent. A coating of
proteins in the tubing and autosampler sometimes
helps to give reproducible results. In some cases
it is good to use a sacrifice protein after
desorb. Why? Desorb removes adsorbed proteins
from the autosampler and the IFC by flushing with
SDS. How? Desorb is run from the menu
Tools-gtWorking tools-gtDesorb. The required
solutions are BIAdesorb solution 1 BIAdesorb
solution 2 These can be found (1) in the laminar
flow bench and (2) in the fridge in the blue
plastic box containing maintenance solutions.
Dock an old sensor chip (from the blue plastic
box containing used sensor chips) into the
Biacore 2000 instrument. Take care to ensure that
the chip is clean before docking. Otherwise the
opto-interface and IFC may be damaged. Prepare
two 11mm glass tubes, one of each containing
solution 1 and 2 respectively. Press start and
follow the instructions on the screen. Note your
run in the yellow log binder. When the solutions
are near exhaustion contact the person
responsible for ordering consumables.
Responsible for this document Olof Andersson
(olle_at_ifm.liu.se, 1214)
2
Biacore 2000 Maintenance protocols
.Sanitize
Who? All users are responsible for running
sanitize during their booking period.
When? Sanitize should be run at least once every
month. If you run biological samples, sanitize
should be run more frequently. For best results,
run desorb before sanitize. Why? Sanitize cleans
the pumps, IFC and autosampler from
micro-organisms. How? Sanitize is run from the
menu Tools-gtWorking tools-gtSanitize. The
required solutions are BIAdisinfectant
solution This can be found in the fridge in
the blue plastic box containing maintenance
solutions. Important This solution needs to be
diluted. Follow instructions on screen. Dock an
old sensor chip (from the blue plastic box
containing used sensor chips) into the Biacore
2000 instrument. Take care to ensure that the
chip is clean before docking. Otherwise the
opto-interface and IFC may be damaged. Note your
run in the yellow log binder. When the solutions
are near exhaustion contact the person
responsible for ordering consumables.
Responsible for this document Olof Andersson
(olle_at_ifm.liu.se, 1214)
3
Biacore 2000 Maintenance protocols
.System check
Who? The person responsible for the Biacore 2000
instrument will run system check when he/she
thinks it necessary. If you experience problems
that you think are caused by an instrument
malfunction you may run system check yourself if
you desire. When? System check is run on a need
to basis. Why? System check can be used to test
for leakage and other malfunctions in the system.
How? System check is run from the menu
Tools-gtTest tools-gtSystem check. The required
solutions are BIAtest solution (sucrose) HBS
buffer These can be found in the fridge in the
blue plastic box containing maintenance
solutions. System check requires a new unused
sensor chip to be docked. The blue plastic box
for maintenance solutions contains a devoted chip
that can be used for system check. Make sure that
the chip is completely clean and dry before
docking. Press start and follow the instructions
on screen. Use the System check evaluation tool
to evaluate the results of the system check. If
you need help to interpret the results contact
the instrument responsible. Note your run and
result in the yellow log binder.
Responsible for this document Olof Andersson
(olle_at_ifm.liu.se, 1214)
4
Biacore 2000 Maintenance protocols
.Shutdown
Who? The current user is responsible for running
shutdown if necessary. When? Shutdown should be
run if the instrument is unbooked for a longer
period, i.e. more than one week. Generally we
only run shutdown during summer and christmas
holidays. Why? Shutdown empties the flow system
and cleans the pumps. If the system is going to
be unused for a longer period this is a necessary
procedure. It also eliminates the need for
continuous flow. How? Shutdown is run from the
menu Tools-gtWorking tools-gtShutdown. The
required solutions are Milli-q water 70
ethanol 70 ethanol can be prepared by mixing 2/3
99.5 ethanol with 1/3 milli-q water. The IFC
can not withstand 70 ethanol, therefore it is
extremely important that you follow the
instructions on the screen carefully. The
connector block to the IFC has to be removed at
the appropriate moment. Note your run in the
yellow log binder.
Connector block screw
Responsible for this document Olof Andersson
(olle_at_ifm.liu.se, 1214)
5
Biacore 2000 Waste disposal
.Waste
Who? All users are responsible for their own
waste. What? The different type of waste
generated in the Biacore 2000 lab
include .Glassware and other hazardous waste
(i.e. pipette tips). .Liquid waste Buffer and
water waste Biochemical and other hazardous
waste .Used vials (plastic and glass) .Used
gloves How? Buffer and water waste can be
disposed of in the sink. Used vials, gloves,
glassware etc. is disposed of in the metal bin
under the desk. When the bin is full it shall be
replaced and disposed of according to the
Handbook of Applied Physics. Any other waste,
especially the liquid waste should be disposed of
by yourself in accordance to current regulations.
You are responsible for keeping a personal waste
bottle. Never leave this bottle in the lab after
your booking is over. Small print If waste
disposal information in any version of the
Handbook of Applied Physics document
contradicts information on this page. The
guidelines in Handbook of Applied Physics
should be followed. It is assumed that all users
are familiar with common waste disposal routines.
Responsible for this document Olof Andersson
(olle_at_ifm.liu.se, 1214)
6
Biacore 2000 Laboratory cleaning
.Cleaning
Who? All users are responsible for leaving the
laboratory in spotless order. When? A thorough
cleaning should be carried out after your booking
session is finished. You are also expected to
keep the laboratory somewhat tidy during your
booking. What? Wipe off all bench-areas
thouroughly with disinfectant. Clean the inner
and outer compartments of the Biacore 2000
instrument. Place everything in its appropriate
place. Dont leave anything unlabelled in the
fridge. When am I finished cleaning? You are
finished when the lab looks like this
Responsible for this document Olof Andersson
(olle_at_ifm.liu.se, 1214)