BiolChem 472

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Biol/Chem 472 Biochemistry!!
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Winter Q 2009 Biol/Chem 472 Metabolism

• Instructor Gerry Prody
• Office CB444
• Office hrs TBA
• gerry_at_chem.wwu.edu
• http//lightning.chem.wwu.edu/dept/facstaff/prody/
  prody-472.htm

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What is Biochemistry?

• the systematic torture of students with copious
  incomprehensible jargon, cryptic fomulae, and
  impossible insoluble problems.

Biochemistry is the study of Life as a process
that can be understood.
Primary Objective understand the molecular
mechanisms that constitute the living state
(Molecular Logic)
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Lehninger Molecular Logic

• A living cell is a self-assembling,
  self-regulating, self-replicating isothermal open
  system of organic molecules operating on a
  principle of maximum economy of parts and
  processes it promotes many consecutive, linked
  organic reactions for the transfer of energy and
  for the synthesis of its own components by means
  of organic catalysts that it produces itself.
• Biol/Chem 471 Biol/Chem 472 Biol/Chem 473

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Elemental composition of the earths surface,
including crust, oceans and atmosphere. Element P
ercent by mass Oxygen 49.1 Silicon 26.1
Aluminum 7.5 Iron 4.7 Calcium 3.4 Sodium
2.6 Potassium 2.4 Magnesium 1.9 Hydrogen
0.88 Titanium 0.58 Chlorine 0.19 Carbon
0.09 All others 0.56
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Table 1-3 Elemental Composition of the Human Body.
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Biol/Chem 472 Expected Outcomes

• draw enzymatic reactions correctly
• correctly calculate DGº and DG for a given step
  or a series of steps in a pathway
• rationalize and/or predict features of pathway
  regulation and describe regulatory mechanisms
• recognize how concentrations of metabolites are
  regulated and the impact that changes in flux
  and/or concentration will have on other
  processes.

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Stryer Fig. 12.30 Fluid Mosaic Model (Nicolson
and Singer)
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Figure 12-20
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Figure 12-37
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Figure 12-45 Secretory Pathway
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Living cells are not at equilibrium!

• Concentrations of reactants and products are
  typically far from the equilibrium values (Q ?
  Keq).
• We must consider steady state concentrations of
  these species for the determination of DG.

DG DGo' RTlnQ
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Homeostatic conditions
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Fig 16.2
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Catabolic pathways
Anabolic pathways
See Figure 16.3
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Figure 16.20
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Figure 16.25

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Table 16.3
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31P NMR of human muscle Before exercise
1 min of exercise
Pi
ATP
phosphocreatine
g a b
19 min of exercise
10 min after exercise
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Reduced substrates
Oxidized substrates


Oxidized cofactors
Reduced cofactors
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Fig 17.3
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Most oxidized
Least oxidized
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Figure 16-21b Some overall coupled reactions
involving ATP. (b) The phosphorylation of ADP by
phosphoenolpyruvate to form ATP and pyruvate.
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Alfonse, Biochemistry makes my head hurt!!
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