MarkinFlanagan Research Project

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Markin-Flanagan Research Project

• Bone Adaptation in a Muscle Disuse Model of Knee
  Osteoarthritic Development
• By Vassiliki Teskey Apr. 26, 2005
• Supervisor Dr. S. K. Boyd
• Dept. of Mechanical and Manufacturing
  Engineering, University of Calgary

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Overview

• Introduction
• Methods
• Scanning Contouring
• Results
• Future Work
• Conclusions

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Introduction
Disease and Joint Injury
Change in Mechanical Environment of Bone
Bone Adapts to Change
Source 1
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Introduction cond

• What is osteoarthritis (OA)?
• Wearing-away of articular cartilage
• Swelling and pain results from degeneration of
  the joint
• Trabecular bone is studied at the part of the
  femur makes up the joint

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Introduction cond

• Used a new model for OA in which a pharmaceutical
  agent (botox) paralysed the muscles around the
  knee
• Why? To distinguish bone changes caused by
• 1. Physiological response
• 2. Changes in mechanical loading

Source 1
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Introduction cond
Source 3 (picture)
Botox (Inject Botox)
Contralateral (Inject Saline)
Control
Source 2
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MicroCT Scanner

• Produces data of sufficient resolution to find
  differences in bone micro-architecture 1

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MicroCT Scanner sample holder
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MicroCT Scanner sample holder
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Rabbit Femur Sample
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Rabbit Femur Sample cond
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Contouring separate bone types
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Contouring zoom-in
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Results Raw xy, Contralateral
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Results Thresholded xy, Contralateral
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Results Raw zx, Contralateral
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Results Thresholded zx, Contralateral
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Results Raw zy, Contralateral
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Results Thresholded zy, Contralateral
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Results Low-resolution Filter
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Results Filter, Contralateral, slice 1
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Results Filter, Contralateral, slice 2
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Results Filter, Contralateral, slice 3
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Results Filter, Contralateral, slice 4
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Future Work

• Contour all samples
• Morphological measurements trabecular thickness,
  separation, number (Tb.Th, Tb.Sp, Tb.N), bone
  volume ratio (BV/TV) and bone surface ratio
  (BS/BV) 1

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Conclusions

• More work must be done.
• Effect of botox on bone micro-architecture is
  unclear from this project.
• GOAL What is the effect of muscle disuse in the
  development of OA in this unique animal model
  1?

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Acknowledgements

• Dr. Steven Boyd, Supervisor
• Dept. of Mechanical and Manufacturing Engineering
• University of Calgary
• Markin-Flanagan Undergraduate
• Student Research Program in Bone
• and Joint Health

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References
1 Boyd, S.K. and Teskey, V.C., 2004.
Application for Markin-Flanagan Winter 2005
Studentship. Undergraduate Student Research
Program in Bone and Joint Health, University of
Calgary. (S.K. Boyd is the author of the project
description, which is what was referenced in this
presentation.)   2 Longino, D., Butterfield,
T.A., and Herzog, W., 2005. Frequency and length
dependent effects of Botulinum toxin-induced
muscle weakness. Journal of Biomechanics 38,
609-613. 3 http//www.awionline.org/lab_animal
s/biblio/la26-4rab.html
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Thank You!
QUESTIONS?