Simulating Urinalysis

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Simulating Urinalysis

• By Rocio Gonzalez

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Introduction

• Do all living things get rid of their wastes
  products?
• Urinary System
• Flow of Urine
• Urinalysis Tests

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Statement of the Problem

• What chemical substances are found in an unknown
  sample of urine?

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Hypothesis

• I believe that there will be glucose, chloride,
  and phosphate in the unknown sample of urine.
• I also dont think that there will be albumin in
  the unknown sample of urine.

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Materials List

• Urine sample with glucose
• Urine sample without glucose
• Urine sample with phosphate
• Urine sample without phosphate
• Urine sample with albumin
• Urine sample without albumin
• Urine sample with chloride
• Urine sample without chloride
• Benedicts solution
• Silver Nitrate

• 12 test tubes
• Glass marking pencils
• Test tube rack
• Test tube holder
• 10 ml graduated cylinder
• Hot plate
• Bunsen Burner
• 400 ml beaker
• Matches
• 10 acetic acid

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Procedure

• PART A TEST FOR GLUCOSE
• 1.) Place two test tubes in a test tube rack.
  Label one test tube G for glucose. Allow the
  other test tube to remain unlabeled as it will be
  the control.
• 2.) Use the 400-mL beaker to prepare a hot water
  bath.
• 3.) Add 3-mL of Benedicts Solution to both test
  tubes
• 4.) Add 3-mL of the urine sample with glucose to
  the test tube labeled G. Then we do the exact
  same thing but for the one with out glucose.
• 5.) Place both test tubes in the hot water bath
  for two minutes.
• 6.) Now you take the test tubes out with a test
  tube holder.
• PART B TEST FOR CHLORIDE
• 1.) Repeat step 1 from above
• 2.) Then we repeat step 3 from above. But it is
  5-mL of urine that we add to the chloride test
  tube.
• 3.) Add 3 drops of silver nitrate solution to
  each test tube

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PROCEDURE CONT

• PART C TEST FOR ALBUMIN
• 1.) Repeat Step 1 from above
• 2.) Half fill the test tube labeled A with the
  urine sample with albumin. Half fill the
  unlabeled test tube with the urine sample without
  albumin.
• 3.) Using a test tube holder, you pass the
  surface of each test tube over a flame of a
  Bunsen burner for 15-20 second. Then we place
  them in a test tube rack.
• 4.) Add 5 drops of acetic acid
• PART D TEST FOR PHOSPHATE
• 1.) Repeat steps 1-4 but on the phosphate test
  tubes.
• PART E TESTING AN UNKNOWN URINE SAMPLE
• 1.) Obtain a sample of artificial urine marked
  unknown.
• 2.) Using four clean test tubes you will perform
  the glucose chloride, albumin, and phosphate
  tests on the unknown sample. Now follow the
  procedures in parts A through D of this
  investigation.

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Data and ObservationsDATA TABLE 1
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Data Table 2
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Data Table 3
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Data Table 4
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Data Table 5
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URINE SAMPLES

• This is a urine sample with a cluster of white
  blood cells
• This is a urine sample with red blood cells and
  bacteria

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• Urine with Red and white blood cells
• Test Tubes Turbidity (left) and normal urine
  (right).

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CONCLUSION

• HYPOTHESIS PARTIALLY CORRECT!
• WHAT HAPPENED IN THIS LAB
• THINGS I LEARNED

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BIBLIOGRAPHY

• Www.science.com
• www.factsofscience.com
• Lab manual and text book

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THE END.