Thanking you for the attention

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Thanking you for the attention
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Participation in SB P1 (NIO)

• WP 0 Project Managements
• WP 1 Mechanisms for establishment of dormant
  stage
• WP 2 Mechanisms for arousal from dormant stages
• WP 4 Engineering/Genetic testing strategies
• WP 5 Discovery of common and divergent pathways
  for dormant stages, dormancy and desiccation
  resistance
• WP 6 Dissemination of results

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Workpackage 1 (WP1) Mechanisms for establishment
of dormant stage
Deliverables Deliverable Number Deliverable date
Optimal conditions for inducing asexually and sexually reproducing rotifers and their resting eggs D4 M9
cDNA libraries, EST sequencing D5 M16
Pattern of global gene expression during formation of resting eggs by miroarray analysis D6 M27
Quantitative analyses of the expression of specific genes by QPCR D7 M35
Proteins and metabolites identified to be associated with formation of resting eggs or contained within them D8 M36
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Workpackage 2 (WP2) Mechanisms for arousal from
dormant stages
Deliverables Deliverable Number Deliverable date
Patterns of global gene expression during exit from dormancy of rotifer resting eggs by microarray analyses D11 M35
Quantitative expression levels of specific genes during resumed development by QPCR D12 M35
Proteins and metabolites associated with resumed metabolic activities during embryonic development in restings eggs and asexual rotifer resting eggs D13 M30
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Workpackage 4 (WP4)Engineering/Genetic testing
strategies
Deliverables Deliverable Number Deliverable date
Differences in the transcriptome, proteome and metabolome analyses between asexual and sexual rotifer strains D20 M35
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Workpackage 5 (WP5)Discovery of common and
divergent pathways for dormant stages, dormancy
and desiccation resistance
Deliverables Deliverable Number Deliverable date
Construction of the databases D24 M30
Identification of species- specific and conserved genes, proteins and compounds D25 M33
Development of in silco models for cell dormancy or desiccation tolerance D26 M36
Production of a comprehensive report D7 M35
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Workpackage 6 (WP6)Dissemination of results
Deliverables Deliverable Number Deliverable date
Participation in scientific meetings D28 M36
A workshop on dormancy opportunities and challenges preservation of cells and organisms D29 M24
Publication of a book on dormancy D30 M36
To be decided at the meeting
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P1- NIO Goals for M6
Goals for M12-16
WP1 Tasks Deliverable Number
Asexual and sexually reproducing rotifer cultures D4 M9
Construction of cDNA libraries (P5) Asexually reproducing rotifers Sexually reproducing rotifers Subtractive library ?? ESTs (P5) 5,000 clones per library (15,000 ESTs for rotifers) D5 M16
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P1- NIO Goals for M12-16
WP2 Tasks Deliverable Number
Collection of asexual rotifer eggs and resting eggs samples for proteome profiling D4 M9
WP5 Tasks Deliverable Number
Compile information on gene and cDNA sequences of rotifers for data base D24 M30