Dr'A'B'Ram Jyothis' M'D Hom

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A Study on Analgesic Activity of Morphinum 4X
and Arnica 3X in Experimental Animal Model

• 
  Dr.A.B.Ram Jyothis. M.D (Hom)
• 
  Lecturer
• Department of
  Homoeopathic Pharmacy
• Fr.Muller Homoeopathic Medical
  College Hospital, Mangalore

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Introduction

• The purpose of fundamental research - to
  understand the phenomena purported by
  Homoeopathy, using the experimental method.
• Experimental method is based on the assumption
  that any hypothesis should be testable, i. e.
  measurements can be done to prove or disprove it.
  

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Contd

• Laboratory research is able to show biological
  activity of Homoeopathic medicines that cannot be
  explained as a placebo response
• This study seeks to test the analgesic effects of
  Morphinum 4X and Arnica 3X on experimental
  animals.

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Objectives

• To evaluate the effects of Homoeopathic potencies
  in Experimental Animal.
• To evaluate the Analgesic Property of Morphinum
  4X and Arnica 3X by Eddys hot plate
  Method.

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Materials and Methods

• Testing Potencies
• Morphinum 4X and Arnica 3X were prepared with Sac
  Lac and Aqua distillata respectively.
• Experimental Animals
• Male albino mice (Swiss strain). Four groups
  (I-IV), each comprising of six animals weighing
  between (20-25g) were selected.

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Contd

• GROUP-I -Control group treated with Aqua
  distillata (10ml/kg, p.o)
• GROUP-II - Group treated with Pentazocine
  (5mg/kg, i.p)
• GROUP III - Group treated with Morphinum 4x
  (10ml/kg, p.o)
• GROUP IV - Group treated with
• Arnica 3x (10ml/kg, p.o)

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Method

• Eddys hot plate Method
• In this method, heat is used as a source of pain.
• Animals are individually placed on a hot plate
  maintained at constant temperature (55?C) and the
  reaction of animals, such as paw licking or jump
  response is taken as the end point.
• Analgesics increase the reaction time.

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Eddys hot plate
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Statistical Analysis

• The results were statistically processed by
  Analysis of variance test (ANOVA) to compare the
  effects on treatment groups before and after
  treatment with control group.
• Dunnets t test were used to determine the effect
  of each treatment against control group.

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Testing of hypothesis

• The null hypothesis (H0)
• No difference in effects of treatments between
  the treatment groups compared with control group.
  
• Alternate hypothesis (H1)
• Significant difference in effect of treatment
  between the treatment groups compared with
  control group.

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Observation

• Control group exhibit no increase in reaction
  time.
• Group treated with Pentazocine exhibit increase
  in reaction time up to 10 sec.
• Group treated with Arnica 3X exhibit increase in
  reaction time up to 6.3 sec.
• Group treated with Morphinum 4X exhibit increase
  in reaction time up to 8.83 sec.

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Result

• The standard drug, Pentazocine (5mg/Kg, i.p)
  increase the reaction time by 65 (Plt0.001).
• Morphinum 4x (10 ml/Kg, o.p) increase the
  reaction time by 49.03 (Plt0.001).
• Arnica 3X (10 ml/Kg, o.p) increase the reaction
  time by 46.51 (Plt0.001).

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Table 1 ANALGESIC EFFECT EDDYS HOT PLATE
Number of animals used in each group
6 The results are in
MeanSEM
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Analysis of variance table for Analgesic activity
before treatment.
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Before Treatment

• The calculated F value is less than table F
  value.
• The null hypothesis is accepted and there is no
  significant difference in the Analgesic activity
  among the groups before treatment.

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Analysis of variance table for Analgesic activity
after treatment.

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After Treatment

• The calculated F value is greater than table F
  value.
• The null hypothesis is rejected and there is
  significant difference in the Analgesic activity
  among the groups after treatment.

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Dunnets t test table against control for
Analgesic activity
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Discussion

• In homoeopathy, Potentized Morphine and Arnica
  are used for unbearable pain.
• Morphinum is indicated in neuralgia where the
  pains are so violent.
• Arnica is indicated when there is sore, lame
  bruised feeling.
• The result of the present study verifies that
  dynamised Morphine and Arnica are potent
  analgesics.

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Conclusion

• The homoeopathic drug, characterized by
  infinitesimal dose has a marked informational
  character.
• The result of the study justify the opportunity
  of future researches concerning the intimate
  mechanism of action of homoeopathic potencies in
  accordance with principle of similia.

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