Snyder Translational Laboratory

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Snyder Translational Laboratory

• Caroline Léger
• May 27, 2009

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The STL lab

• Room HSC 2541
• Director Caroline Léger
• Research assistant Sarah Pineau
• Hours of operation 8h30am to 4h30pm
• Expertise in
• Luminex technology
• flow chamber assay
• molecular biology techniques

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How does the Luminex works?

• Mix between Elisa and FACS

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How are the readings done?
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Luminex

• Able to process from 5 to 42 analytes at once
• Multiple sample sources
• Plasma, serum, CSF, BAL, urine, brain
  microdialysate
• Cell culture supernatant
• protein extracts
• Able to compare
• Patients groups
• Sample type
• Time course
• Determine grouped pattern of expression

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What are the benefits?

• Eliminate inter-assay variation
• Help preserve valuable samples
• For a 25 plex 25 ?l for luminex vs 1250 ?l for
  25 Elisa
• Reduced labor and cost
• for a 25 plex 1 day with luminex vs 12 days with
  Elisa
• Multiformat platform
• Quantitative, semi-quantitative, qualitative

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How this service will work?

• You buy the kit
• You can perform the experiment in the Snyder
  Translational Lab using our plate shaker and
  plate washer with appointment
• We enter the info in the computer and run the
  plate for you on the Luminex
• We transfer your data to Excel and send it to you

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What else can we help you with?

• development and troubleshooting of protocols
• In house Luminex assays
• Flow chamber or molecular biology techniques
• animal work information
• put you in touch with clinicians in ICU with
  common interests
• more! Just contact Sarah or Caroline.

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On the website, we present

• tutorials on the different techniques used in the
  laboratory
• the CCCTBG approved SOPs for the isolation of
  plasma, serum, urine, etc
• summary of what we can do and what are the
  different techniques
• list of publication of the members
• contact information

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Snyder Chair Website

• http//www.ucalgary.ca/snyder_chair

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More questions? Just contact Sarah or Caroline

• Thank you!