Susan Ceryak, Ph.D.

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Survival signaling and carcinogenesis
Susan Ceryak, Ph.D. Associate Research
Professor Departments of Pharmacology
Physiology and of Medicine Tel 202 994
3896 phmsmc_at_gwumc.edu
NIH/NCI R01CA107972 McCormick Pilot Grant
January 10, 2007
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Overall objective to elucidate the coordinate
signaling events mediating cell fate
determination and survival after genotoxic insult
Can we alter cell fate after genotoxin exposure
by manipulating survival pathways?
Do survivors of genotoxin exposure exhibit
altered survival pathways?
NIH/NCI R01CA107972 McCormick Pilot Grant
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BJ-hTERT
Human diploid fibroblasts
genotoxin
Parental (Death-sensitive)
Death Resistant DP 5.14
genotoxin
Subcloned from survivors of genotoxin
exposure Altered survival signaling
Death-resistant
Laura Beaver
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After genotoxin exposure in death resistant cells
AKT is upregulated
VDAC is NOT upregulated
Mitochondrial HK II association is enhanced
Mitochondrial reticular network is altered
Laura Beaver Therese Lizardo Kristen
Wright Ashley Larrimore
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Hypothetical model for AKT-mediated death
resistance
Mitochondrion
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Cr(VI) PTP inhibitor (SOV)
Is AKT necessary for SOV effect? siRNA/ dn AKT
studies? What other pathways are involved in SOV
effect? (gene array) What is the mechanism of
cell cycle checkpoint bypass?
Dongsoon Bae Tura Camilli
Gina Chun Kristen Wright