Lipase with enhanced activity

1
Lipase with enhanced activity

• A possible supplementary therapy for pancreatic
  insufficiency

2
Human pancreatic lipase
3
Lipolysis

• Lipase is converting triaglycerides into fatty
  acids and monoglycerol.

4
Industrially use of lipase

• Food industry
• Detergents
• Production of chemicals
• Production of pharmaceuticals
• Fungicide
• etc.

5
Objective

• Finding an alternative supplementary therapy for
  patients with a poor fat absorption due to an
  insufficient or complete loss of pancreatic
  lipase.

6
Why?

• Lipid digestion is not completely restored by
  currently used therapies.
• Hypersensitivity towards porcine lipase
• Religion

7
Methods

• Isolation of gene from suitable organism
• Cloning into E. coli
• Random mutations
• Screening
• Activity measurements
• Expression of protein
• In vivo test
• Up scaling?