How is a single cell, the egg, patterned

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How is a single cell, the egg, patterned?
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Outline for Lecture

• Techniques
• Oogenesis
• selecting oocyte
• Patterning the oocyte
• Dorsal/ventral patterning
• Anterior/posterior patterning

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Techniques

• Chromosomes Balancers
• multiple inversions
• recessive lethal
• dominant marker
• Example CyO

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Techniques
P elements GMR-Retactivated
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Techniques
Genetic modifier screen
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Techniques
FRT/FLP STAT (clone)
Anti-Stat92E
GFP
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Techniques
FRT/FLP CSK (EGUF)
Wild type control
dCskP244 EGUF clone
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Oogenesis Oogenesis Oogenesis Oogenesis
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Oogenesis Oogenesis Oogenesis Oogenesis Oogenesis
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Oogenesis Oogenesis Oogenesis Oogenesis
Oogenesis Oogenesis Oogenesis Oogenesis
Oogenesis Oogenesis Oogenesis Oogenesis Oogenesis
Oogenesis Oogenesis Oogenesis Oogenesis Oogenesis
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Oogenesis Defining the Oocyte within the Germarium
Adapted from Navarro et al, 2001, Buskirk and
Schupbach, 1999, van Eeden and St. johston, 1999
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Oogenesis Patterning the Oocyte
The egg chamber nurse cells oocyte
follicle cells
Adapted from Reichman and Ephrussi, 2001 Buskirk
and Schupbach, 1999
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Oogenesis Patterning the Oocyte
Patterning follicle cells oocyte Gurken
expression in blue (green) Gurken induces fc
posterior fate follicle cells (T)
terminal fcs posterior fcs activate EGFR
Adapted from Reichman and Ephrussi, 2001 Buskirk
and Schupbach, 1999
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Oogenesis Patterning the Oocyte
Dorsal/Ventral identity Nucleus moves to an
anterior corner uses microtubules,
Gurken to move Wherever it sits, that defines
the dorsal side of the oocyte Gurken is
associated with nucleus (green)
Adapted from Reichman and Ephrussi, 2001 Buskirk
and Schupbach, 1999
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Oogenesis Patterning the Oocyte
Anterior/Posterior identity Bicoid and Oskar
are dumped into the oocyte through ring canals
of nurse cells. They localize to different parts
of the oocyte and define the A/P axis.
We'll talk more about this on Thursday
Adapted from Reichman and Ephrussi, 2001 Buskirk
and Schupbach, 1999
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Oogenesis Overview
Stage 10 D/V axis
Stage 9 A/P axis
Dpp, Gurken Induction of dorsal cell fates
bicoid mRNA oskar mRNA
Pipe
Stage 14 patterned oocyte
Dorsal appendages
micropile
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For next Tuesday (one or both) http//flymove.un
i-muenster.de Arnosti, Barolo, Levine, and
Small (1996) Development 122,205-214
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