Lab 3 Goals and Objectives:

1
Lab 3 Goals and Objectives Exercise 7 Ubiquity
of Bacteria Observe results Exercise 9 Aseptic
Technique Check results Exercise 10 Pure
Culture Technique Check results, repeat once
from your plate if necessary Red S. marcescens
/ Small yellow M. luteus / Cream E.
coli Exercise 11 Smear Preparation Each person
make smear slides Less is More!!!! Your
broth E. coli (from Ex. 9), Your slant E. coli
(from Ex. 9) Interesting colonies (2-5) from
home plates (Exercise 7) Exercise 12 Simple
Staining Each person stain slides from Exercise
11 (No Corynebacterium) All stain and rinse
water into tray nothing down sink!!! Hint for
microscopy Best smears will be barely visible
to the naked eye when complete. In order to find
the focal plane put a marker dot on the finished
dry slide, same side as the bacteria, before
going to the scope and use the dot to find your
focus. Label a slide box with your name on tape
and store any slides you wish to save. Blot oil
off for storage. No methanol on slides (removes
sample) !!!CLEAN YOUR SCOPE WELL!!!
2
Coccus
Bacterial Shapes
Lab book pg 55 Sup. packet pg 56
Cocci Diplococci Tetrad Streptococci Staphylococci
Bacillus
Bacilli Coccobacilli Streptobacilli Fusiform
Spiral
Vibrio Spirillum Spirochaetes
3
Figure 11.1
Water first always with solid media
Target circle optional spread out wide to
make smear as thin as possible
No big blobs!
Must be COMPLETELY dry!
4
Figure 12.1
paper towel
5
Lab 3 Goals and Objectives Exercise 7 Ubiquity
of Bacteria Observe results Exercise 9 Aseptic
Technique Check results Exercise 10 Pure
Culture Technique Check results, repeat once
from your plate if necessary Red S. marcescens
/ Small yellow M. luteus / Cream E.
coli Exercise 11 Smear Preparation Each person
make smear slides Less is More!!!! Your
broth E. coli (from Ex. 9), Your slant E. coli
(from Ex. 9) Interesting colonies (2-5) from
home plates (Exercise 7) Exercise 12 Simple
Staining Each person stain slides from Exercise
11 (No Corynebacterium) All stain and rinse
water into tray nothing down sink!!! Hint for
microscopy Best smears will be barely visible
to the naked eye when complete. In order to find
the focal plane put a marker dot on the finished
dry slide, same side as the bacteria, before
going to the scope and use the dot to find your
focus. Label a slide box with your name on tape
and store any slides you wish to save. Blot oil
off for storage. No methanol on slides (removes
sample) !!!CLEAN YOUR SCOPE WELL!!!