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Promoters for the 3 nuclear RNA polymerases nRNAPs PowerPoint PPT Presentation

Promoters for the 3 nuclear RNA polymerases nRNAPs - ... mutagenesis of the Herpes virus tk promoter identifies 2 important upstream regions. ... Flip 180 degrees, no effect. Both are position independent. Can ...

... mutagenesis of the Herpes virus tk promoter identifies 2 important upstream regions. ... Flip 180 degrees, no effect. Both are position independent. Can ...

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Monocistronic vs Polycistronic Genes PowerPoint PPT Presentation

Monocistronic vs Polycistronic Genes - Isolated Spinach chloroplast envelope thylakoid membrane stroma From Hoober Chloroplast RNA polymerases (RNAPs) Two different RNAPs in vascular plant chloroplasts ...

Isolated Spinach chloroplast envelope thylakoid membrane stroma From Hoober Chloroplast RNA polymerases (RNAPs) Two different RNAPs in vascular plant chloroplasts ...

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The Yeast nRNAP II PowerPoint PPT Presentation

The Yeast nRNAP II - Gene knockouts indicate that 10 subunits are essential, & 2 are required under ... have regions of homology with the b and b' subunits of the E. coli RNAP and ...

Gene knockouts indicate that 10 subunits are essential, & 2 are required under ... have regions of homology with the b and b' subunits of the E. coli RNAP and ...

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Transcription in the Eukaryotic Nucleus PowerPoint PPT Presentation

Transcription in the Eukaryotic Nucleus - Actinomycin D - general transcription inhibitor, binds DNA and intercalates into ... Actinomycin D, at low concentrations, did not inhibit synthesis of heterogenous ...

Actinomycin D - general transcription inhibitor, binds DNA and intercalates into ... Actinomycin D, at low concentrations, did not inhibit synthesis of heterogenous ...

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Outline - Factors RNAP must also bind in a specific order (in vitro) ... TFIIIB binds to TFIIIC, enabling TFIIIB to bind upstream and recruit the Pol ...

Factors RNAP must also bind in a specific order (in vitro) ... TFIIIB binds to TFIIIC, enabling TFIIIB to bind upstream and recruit the Pol ...

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Transcription Factors for Class II promoters RNAP II PowerPoint PPT Presentation

Transcription Factors for Class II promoters RNAP II - Promote transcription from some class II promoters that lack a TATA box. ... Pre-initiation complexes on TATA-less promoters contain TBP. ...

Promote transcription from some class II promoters that lack a TATA box. ... Pre-initiation complexes on TATA-less promoters contain TBP. ...

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Transcription Factors for Class II promoters RNAP II - Required for initiation at nearly all promoters; determine site of initiation; ... Work like upstream factors but are regulatory. Made or active only at ...

Required for initiation at nearly all promoters; determine site of initiation; ... Work like upstream factors but are regulatory. Made or active only at ...

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Transcription in the Eukaryotic Nucleus - RNA synthesis by isolated nuclei indicated that there were at least 2 ... 3 nuclear RNA polymerases from sea urchin embryos by ion exchange chromatography ...

RNA synthesis by isolated nuclei indicated that there were at least 2 ... 3 nuclear RNA polymerases from sea urchin embryos by ion exchange chromatography ...

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The Yeast nRNAP II - ... have regions of homology with the b and b' subunits of the E. coli RNAP and ... Result: It required deleting more than 50 bp into this small gene to knock out ...

... have regions of homology with the b and b' subunits of the E. coli RNAP and ... Result: It required deleting more than 50 bp into this small gene to knock out ...

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Class I Promoters for nRNAP I PowerPoint PPT Presentation

Class I Promoters for nRNAP I - Class I Promoters (for nRNAP I) Sequences less conserved than Class II ... deletions at the 5'-end of the Xenopus 5S rRNA gene on its transcription in vitro. ...

Class I Promoters (for nRNAP I) Sequences less conserved than Class II ... deletions at the 5'-end of the Xenopus 5S rRNA gene on its transcription in vitro. ...

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Transcription in the Eukaryotic Nucleus - Actinomycin D - general transcription inhibitor, binds DNA and intercalates into ... Not inhibited by aminitin, but inhibited by low concentrations of actinomycin D. ...

Actinomycin D - general transcription inhibitor, binds DNA and intercalates into ... Not inhibited by aminitin, but inhibited by low concentrations of actinomycin D. ...

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Factors for Initiation at Class I Promoters PowerPoint PPT Presentation

Factors for Initiation at Class I Promoters - Acidic (e.g., GAL4, 49 aa domain 11 acidic aa) Glutamine-rich (e.g., 2 in Sp1, ~25% gln) ... Modules with 2 Zinc ions and 6 cysteines (GAL4) ...

Acidic (e.g., GAL4, 49 aa domain 11 acidic aa) Glutamine-rich (e.g., 2 in Sp1, ~25% gln) ... Modules with 2 Zinc ions and 6 cysteines (GAL4) ...

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Nrnaps PowerPoint PPT Presentations