Prehybridized at 60 C in seal-a-meal bag to mask free potential DNA binding site ... on membrane to determine which restriction fragment(s) may harbor the Myb61 gene ...
Probe Classification: Heterologous- from a different organism ... with a solution of single stranded Myb61 probe ... Wash to eliminate unbound probe molecules ...
Agarose Gel Electrophoresis and Southern Transfer Broad and Long Term Objective Research Plan Today s Laboratory Objectives To become familiar with a Southern ...
Digest Genomic DNA w/ Various Restriction Enzymes ... 1. To Isolate High Quality Genomic DNA ... Small flowering plant used as model organism in plant biology ...