Study and engineering of gene function: mutagenesis Why mutagenize? Random mutagenesis, mutant selection schemes Site-directed mutagenesis, deletion mutagenesis
Digest Final PCR product with Hind III and Kpn I; isolate 474 bp fragment ... EtOH precipitated, gel-pure vectors (digested with Hind III and Kpn I) DCLD. DB 1 2 ...
... Julien Guy, Nicolas Regnault, Christophe Balland, Delphine Hardin, Jim Rich, ... Ray Carlberg, Alex Conley, Andy Howell, Kathy Perrett, Mark Sullivan. The ...